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SIMPLE PCR TECHNIQUE FOR DETECTING AND DIFFERENTIATING BACTERIAL PATHOGENS

A simple polymerase chain reaction procedure is described for the detection and differentiation of Shigella from other pathogenic Escherichia coli isolates, such as EIEC and EPEC. Serotype specific primers derived from the rfc genes of different Shigella strains are used to identify the most prominents Shigella serotypes, such as S. sonnei, S. flexneria 1 through 5, and S dysenteriae 1. More than 95% of Shigellosis cases reported could be identified by the serotype specific primers described.


Docket:WRAIR 96-09

Publication/Issued No.:5,958,686

Publication/Issue Date:1999-09-28

Categories: Assay

More Detail:Visit USPTO.GOVExternal link

Lab:WRAIR

Inventor(s):HOUNG, H.

Abstract:Download


For Licensing Opportunities, please contact the ORTA at USArmy.Detrick.MEDCOM-USAMRMC.List.ORTA@mail.mil
 

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